Rabbit anti Swine IgG (Fc specific), conjugated with Horseradish peroxidase

Rabbit anti Swine IgG (Fc specific), conjugated with Horseradish peroxidase, Polyclonal, Clone: Polyclonal

Clone: Polyclonal

Background: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal swine plasma and serum, a single characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line obtained with purified IgG. It does not react with any non-Ig protein in swine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Source: Purified normal IgG isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Swine IgG, Fc specific.

Formulation: Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Artikelnummer: RASw/IgGFc/PO
• Hersteller: Nordic-MUbio
• Hersteller Artikelnummer: RASw/IgG(Fc)/PO
• Green Labware: Nein
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Pig (Porcine)
• Klonalität: Polyclonal
• Methode: Immunohistochemistry (paraffin), Western Blotting, ELISA, Immunocytochemistry, Dot Blot
• Wirt: Rabbit