This product is used as red blood cell (RBC) lysis buffer and is supplied as a 10X solution. It should be diluted to 1X in deionized water before using. The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. Samples must be prepared as single cell suspension in an appropriate anticoagulant (EDTA is recommended) tube.This product is used as red blood cell (RBC) lysis buffer and is supplied as a 10X solution. It should be diluted to 1X in deionized water before using. The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. Samples must be prepared as single cell suspension in an appropriate anticoagulant (EDTA is recommended) tube.