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Encompassing Amino Acids: Rbx1: 2-108(end); CUL4B: 3-913; DDB1: 2-1140(end); DCAF15: 2-600(end); DDA1: 2-102(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to DCAF15 TR-FRET Assay Kit with DCAF15 titrated up to 50 nM.
Description: Recombinant Recombinant human complex of RBx1 (ring-box 1), CUL4B (cullin 4B), DBB1 (damage specific DNA binding protein 1), DCAF15 (DDB1 and CUL4 associated factor 15), and DDA1 (DET1 and DDB1 associated 1). RBx1, full length, encompassing amino acids 2-108(end). This construct contains an N-terminal His-tag (6xHis). CUL4B, encompassing amino acids 3-913(end). This construct contains an N-terminal His-tag (6xHis). DBB1, full length, encompassing amino acids 2-1140(end). This construct contains an N-terminal Flag-tag. DCAF15, full length, encompassing amino acids 2-600(end). This construct contains an N-terminal Flag-tag. DDA1, full length, encompassing amino acids 2-102(end). This construct contains an N-terminal Flag-tag. These recombinant proteins are co-expressed in a HEK293 expression system and affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol
Genbank: DDB1: NM_001923; CUL4B: NM_003588.4; DCAF15: NM_138353.4; DDA1: NM_024050.6; RBX1: NM_014248
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-Avi-Tag, N-terminal His-Tag
Uniprot: DDB1: Q16531; CUL4B: Q13620; DCAF15: Q66K64; DDA1: Q9BW61; RBX1: P62877
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)