Recombinant Human CDK2 Protein (E.coli, His Tag)

Abbreviation: CDK2

Target Synonym: Cyclin-Dependent Kinase 2;Cell Division Protein Kinase 2;p33 Protein Kinase;CDK2;CDKN2

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His

UNIProt ID: P24941

Background: Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.

Sequence: Met 1-Leu298

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% tween-80.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.

• Artikelnummer: ELSPDEH100009-20
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-PDEH100009-20
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK