Protein Tag: N-Sumo
Uniprot: P02775
Accession: P02775
Background: Pro-platelet basic protein (PPBP) is also known as Chemokine (C-X-C motif) ligand 7 (CXCL7) and nucleosome assembly protein (Nap-2). Nap-2 / PPBP / CXCL7 is released in large amounts from platelets following their activation and is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. Nap-2 / PPBP / CXCL7 has been shown to stimulate various cellular processes including DNA synthesis , mitosis , glycolysis , intracellular cAMP accumulation , prostaglandin E2 secretion , and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Nap-2 is a ligand for CXCR1 and CXCR2 , and Nap-2 , Nap-2 (73) , Nap-2 (74) , Nap-2 (1-66) , and most potent Nap-2 (1-63) are chemoattractants and activators for neutrophils.
Bio Acitivity: Not validated for activity
Sequence: Ala59-Asp128
Purity: > 90% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.
Calculated MW: 20.59 kDa
Observed MW: 25 kDa
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