Recombinant Human DNase-1 protein (His tag)

Abbreviation: DNase-Ⅰ

Target Synonym: DNL1;DRNI;DNL1;Deoxyribonuclease-1;DNase I

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His

UNIProt ID: P24855

Background: DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.

Sequence: Leu 23-Ala 259

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Endotoxin: Please contact us for more information.

• Artikelnummer: ELSPDEH100176-20
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-PDEH100176-20
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK