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Recombinant Rat Epidermal Growth Factor/EGF protein (His tag)

Recombinant Rat Epidermal Growth Factor/EGF protein (His tag)
Artikelnummer
ELSPDER100178-100
Verpackungseinheit
100μg
Hersteller
Elabscience Biotechnology

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Special promotion: Enjoy a 10% discount on Elabscience recombinant proteins through September 30, 2025.
Promo code: ELABPQ3
No additional discounts available during this period.

Protein Tag: N-His & C-His

Uniprot: P07522

Accession: P07522

Background: EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 645-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell-a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR-that ultimately lead to DNA synthesis and cell proliferation.

Bio Acitivity: Not validated for activity

Sequence: Gln 22-Leu 154

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.

Calculated MW: 14.5 kDa

Observed MW: 16 kDa
Mehr Informationen
Artikelnummer ELSPDER100178-100
Hersteller Elabscience Biotechnology
Hersteller Artikelnummer PDER100178-100
Verpackungseinheit 100μg
Mengeneinheit STK
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