Recombinant Swine FGF-2 protein(His Tag)

Protein Tag: N-His

Uniprot: P03969

Accession: NP_001392443

Background: Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial; tumor and other cell types. It involved in developmental processes and regulates differentiation; proliferation; and migration; FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.

Bio Acitivity: Measure by its ability to induce proliferation in 3T3 cells. The ED₅₀ for this effect is <2 ng/mL.

Sequence: Ala 2-Ser 155

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS with 0.01% sarkosyl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.1 kDa

Observed MW: 17 kDa

• Artikelnummer: ELSPKSS000016-5
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: PKSS000016-5
• Verpackungseinheit: 5μg
• Mengeneinheit: STK
• Methode: Cell Culture