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Encompassing Amino Acids: 16-685
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay run according to the Spike S1 (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit (BPS Bioscience #78358). 5 ng of ACE2-Eu, 400-fold diluted Dye-labeled acceptor, and various amounts of Spike S1[B] incubated for 1 hour at room temperature. TR-FRET measured at ex340 ± 20nm, em620 ± 10 nm; and ex340 ± 20nm, em665 ± 10 nm.
Description: Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 16-685. This protein corresponds to SARS-CoV-2 Variant B.1.1.529 BA.1, also known as variant Omicron BA.1 originally identified in South Africa, and contains genetic alterations indicated below. It was constructed with a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Genbank: MN908947
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Avi-His-tag
Uniprot: P0DTC2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)