StemBright™ Luciferase iPS Cell Pool

StemBright™ Luciferase iPS Cell Pool
Artikelnummer
BPS78594
Verpackungseinheit
1 vial
Hersteller
BPS Bioscience

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Products from BPS Bioscience require a minimum order value above 400€

Applications: In vitro cell viability assaysIn vitro co-culture assaysMonitor iPS derived cell localization in vivo using bioluminescent imaging (BLI)

Background: The discovery by Yamanaka and colleagues in 2007 that 4 factors were sufficient to reprogram terminally differentiated fibroblasts into pluripotent stem cells launched the advent of human induced pluripotent stem (iPS) cell technology. These human iPS cells are capable of both self-renewal and differentiation down all three germline lineages and provide both a tool to model human development and disease in the relevant differentiated human cell types, and a unique opportunity for high throughput drug screening.

Description: The StemBright™ Luciferase iPS Cell Pool constitutively expresses the firefly luciferase gene under the control of a CMV promoter, introduced via lentiviral transduction (Firefly Luciferase Lentivirus, BPS Bioscience #79692). Luciferase activity can be used to monitor cell viability, both in vitro and in vivo.

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.As this is a cell pool and not a cell line, BPS Bioscience cannot guarantee the stability of the genetic modifications over time. Clonal selection can be performed. We recommend freezing cell vials very early on and growing the cells for a limited number 

Supplied As: Each vial contains 2 x 106 cells in 1 ml of iPS cell Freezing Medium

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

References: Cao F, et al., 2006 7:1005-1014.Lepperhof V, et al., 2014 PLoS One. 9(9):Li X, et al., 2015 Int J Clin Exp Med. 8(6):8938-8947.Liu Z, et al., 2013 PLoS One. 8(6):Nishimura, et al., 2022 Methods in Molecular Biology. 2524:291-297.Takahashi K, et al., 2007 131(5):861-872.
Mehr Informationen
Artikelnummer BPS78594
Hersteller BPS Bioscience
Hersteller Artikelnummer 78594
Verpackungseinheit 1 vial
Mengeneinheit STK
Wirt Human
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