T4 β-glucosyltransferase, His-tag Recombinant

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Encompassing Amino Acids: 1-351(end)

Application: Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

Description: T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, Genbank Accession No. NP_049658, a.a. 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Genbank: NP_049658

Purity: 0,83

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: P04547

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Song, C.X., et al. (2011). Nat Biotechnol. 29(1): 68-72.
2. Kornberg, S.R., et al. (1961). J. Biol. Chem. 236: 1487-1493.

• Artikelnummer: BPS72000-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 72000-1
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK