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Encompassing Amino Acids: 279-390(end)
Application: Useful for cell culture and for the study of signaling and apoptotic pathways.
Biological Activity: The ED50 measured by TGFβ1's ability to inhibit mIL-4-dependent proliferation of HT-2 mouse helper T cells is <0.05ng/ml.
Description: Human TGFb1 (GenBank Accession #NM_000660), a.a. 279-390(end), disulfide-linked homodimer. MW= ~25 kDa. Ths is the mature active form of the protein.
Endotoxin Level: < 1EU per µg
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered, aqueous solution
Genbank: NM_000660
Host Cell Line: FreeStyle 293-F cells
Reconstitution: Reconstitute in sterile 0.2 M acetic acid with 0.1% BSA. Further dilutions may be made in PBS with 0.1% BSA. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: >6 months at -80°C (as a solid). Store reconstituted protein in aliquots at -80°C for up to 3 months. Avoid freeze/thaw cycles.
Tags: none
Uniprot: P01137
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Derynck, R. et al. (1985). Nature 316(6030):701-5.
2. Bourdrel L., et al. (1993). Protein Expr. Purif. 4:130-140.
3. Munger, J.S., et al. (1997). Kidney Int. 51:1376-1382.