Description: Sandwich TR-FRET immunoassay for quantitative detection of human IgG Fc in cell culture supernatants. The kit does not include a recombinant human IgG standard, which must be purchased separately from Sigma-Aldrich ® (catalog number I4506).
Components: Eu-anti-hIgG Fc (25 µL), Acc-anti-hIgG Fc (100 µL), 5X Assay Buffer 1 (3 mL).
Assay Type: Quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Human IgG.
Assay Target Class: Human IgG, Fcγ fragment specific.
Detection method: TR-FRET.
Sample type: Cell supernatants.