Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total 4EBP1 in cell lysates.
Components: Eu-Total AKT (250 µL), Acc-Total AKT (1 mL), AKT control lysate (2 x 200 µL), 5X Lysis Buffer 1 (50 mL), 10X TR-FRET Detection buffer (2.5 mL), 100X Phosphatase Inhibitor Cocktail (2.5 mL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Total 4EBP1.
Assay Target Class: Kinase.
SwissProt: Q13541.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).