Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total BTK in cell lysates.
Components: Eu-Total BTK (5 µL), Acc-Total BTK (20 µL), BTK control lysate (100 µL), 5X Lysis Buffer #2 (1 mL), 10X TR-FRET Detection buffer (50 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Total BTK.
Assay Target Class: Kinase.
SwissProt: Q06187.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).