Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total 4EBP1 in cell lysates.
Components: Eu-Total 4EBP1 (5 µL), Acc-Total 4EBP1 (20 µL), 4EBP1 control lysate (100 µL), 5X Lysis Buffer 2 (1 mL), 10X TR-FRET Detection buffer (50 µL), 100X Phosphatase Inhibitor Cocktail (50 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Total 4EBP1.
Assay Target Class: Kinase.
SwissProt: Q13541.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).