UBE1 (UBA1), FLAG-tag Recombinant

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Encompassing Amino Acids: 2-end

Application: Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay Conditions: Assay was done according to UBE1 Inhibitor Screening Assay Kit (BPS Bioscience #79957) with UBE1 titrated from 1500 ng/rxn to 0 ng/rxn.

Description: Human UBE1 (GenBank Accession No. NM_003334), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol

Genbank: NM_003334

Host Cell Line: Sf9 cells

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag

Uniprot: P22314

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008).
2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).

• Artikelnummer: BPS80301-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80301-1
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Insect