UBE1 (UBA1), FLAG-tag Recombinant

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Encompassing Amino Acids: 2-1058(end)

Application: Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay Conditions: Assay was done according to UBE1 Inhibitor Screening Assay Kit (BPS Bioscience #79957) with UBE1 titrated from 1500 ng/rxn to 0 ng/rxn.

Description: Human Ubiquitin Activating Enzyme (E1) (UBE1), also known as UBA1, GenBank Accession No. NM_003334, a.a. 2-1058(end), with N-terminal FLAG-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=119 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 80 µg/ml FLAG peptide.

Genbank: NM_003334

Host Cell Line: Sf9 cells

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal FLAG-tag

Uniprot: P22314

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008).
2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).

• Artikelnummer: BPS80301-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80301-2
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Wirt: Insect