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Encompassing Amino Acids: 2-76
Applications: Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
Description: Human Ubiquitin, also known as UBB, UBC, UBA52, and RPS27A. GenBank Accession No. NM_021009, a.a. 2-76, with C-terminal His-tag, expressed in an Sf9 expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, Variable imidazole, and 20% glycerol
Genbank: NM_021009
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: C-terminal His-tag
Uniprot: P0CG48
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Bodine, S., et al., Science. 2001; 294(5547): 1704-1708.2. Bence, N., et al., Science. 2001; 292(5521): 1552-1555.