English
USP13, His-Tag, FLAG-Tag Recombinant
USP13, His-Tag, FLAG-Tag Recombinant
Artikelnummer
BPS102018-1
Verpackungseinheit
25 µg
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-863(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: USP13 was incubated in 50 mM Tris-HCl, 0.5 mM EDTA, 0.05% Tween 20, 1 mM DTT with 500 nM Ubiquitin-AMC. The reaction was incubated at room temperature for 30 minutes. Fluorescence intensity was measured at λexc/λem of 360nm/460nm using a Tecan M1000 plate reader.
Background: USP13 (ubiquitin carboxyl-terminal hydrolase 13), also known as isopeptidase T 3 (ISOT-3) is a DUB (deubiquitination) enzyme. It participates in multiple crucial cellular processes, such as autophagy, DNA damage response, ERAD (endoplasmic reticulum-associated degradation) by deubiquitinating proteins involved in those functions. It also plays roles in diseases such as cancer and neurodegeneration. It can play both a suppressor and activator role in tumorigenesis. For instance, low levels of USP13 correlate with low levels of PTEN (phosphatase and tensin homolog), as UPS13 stabilizes the protein. A deeper understanding of all its cellular roles and how to best target its function in a specific context may prove beneficial in cancer therapy.
Description: Recombinant human USP13 (ubiquitin carboxyl-terminal hydrolase 13), encompassing amino acids 2-863(end). This construct contains an N-terminal His-tag (6xHis) and a
C-terminal FLAG-tag. This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.48 mM KCl, 0.045% Tween-20, 10% glycerol, 100 µg/ml FLAG peptide
Genbank: NM_003940.3
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag, C-terminal FLAG-Tag
Uniprot: Q92995
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
Encompassing Amino Acids: 2-863(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: USP13 was incubated in 50 mM Tris-HCl, 0.5 mM EDTA, 0.05% Tween 20, 1 mM DTT with 500 nM Ubiquitin-AMC. The reaction was incubated at room temperature for 30 minutes. Fluorescence intensity was measured at λexc/λem of 360nm/460nm using a Tecan M1000 plate reader.
Background: USP13 (ubiquitin carboxyl-terminal hydrolase 13), also known as isopeptidase T 3 (ISOT-3) is a DUB (deubiquitination) enzyme. It participates in multiple crucial cellular processes, such as autophagy, DNA damage response, ERAD (endoplasmic reticulum-associated degradation) by deubiquitinating proteins involved in those functions. It also plays roles in diseases such as cancer and neurodegeneration. It can play both a suppressor and activator role in tumorigenesis. For instance, low levels of USP13 correlate with low levels of PTEN (phosphatase and tensin homolog), as UPS13 stabilizes the protein. A deeper understanding of all its cellular roles and how to best target its function in a specific context may prove beneficial in cancer therapy.
Description: Recombinant human USP13 (ubiquitin carboxyl-terminal hydrolase 13), encompassing amino acids 2-863(end). This construct contains an N-terminal His-tag (6xHis) and a
C-terminal FLAG-tag. This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.48 mM KCl, 0.045% Tween-20, 10% glycerol, 100 µg/ml FLAG peptide
Genbank: NM_003940.3
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag, C-terminal FLAG-Tag
Uniprot: Q92995
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS102018-1 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 102018-1 |
| Verpackungseinheit | 25 µg |
| Mengeneinheit | STK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
