3CL Protease, MBP-tag, His-tag (MERS-CoV) Recombinant

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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Add 40 µl of MERS-CoV 3CL protease diluted in 1x assay buffer. Start the reaction by adding 10 µl of diluted substrate. Incubate 4 hours at 37°C. Read fluorescence (λexc=485 nm, λem=528 nm). Total volume is 50 µl.

Description: Middle East Respiratory Syndrome Coronavirus 3C-like protease (MERS-CoV 3CL Protease), GenBank Accession No. ATG84821.1, a.a. 1-306(full length), with an N-terminal MBP-tag, and C-terminal His-tag, expressed in an E. coli expression system, MW=78 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 20% glycerol, variable Imidazole.

Genbank: ATG84821.1

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal MBP-tag, C-terminal His-tag

Uniprot: A0A291I686

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Jo, S. et al., J. Enzyme Inh. & Med. Chem. 2019; 1(35): 145-151.
2. He, J. et al., Intl J. Antimicrobial Agents. 2020; doi.org/10.1016/j.ijantimicag.2020.106055.

• Artikelnummer: BPS100757-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100757-1
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK