3CL Protease (MERS-CoV) Inhibitor Screening Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Background: Middle East Respiratory Syndrome coronavirus (MERS-CoV) poses a significant threat to public health worldwide due to its ability to cause serious human disease with high mortality rates. MERS-CoV has become a global threat due to continuous outbreaks in countries on the Arabian Peninsula and the potential for spread to other countries. MERS-CoV 3CL protease is essential for viral replication; consequently, it is an attractive target for small molecule therapeutics.

Contraindications: The final concentration of DMSO in the reaction should be ≤1%.

Description: The 3CL Protease (MERS-CoV) Inhibitor Screening Assay Kit is designed to measure 3CL Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. A protease inhibitor cocktail is included as a positive control. Assay Principle: 3CL Protease (MERS-CoV) Assay Kit uses a custom-synthesized peptide which has a fluorescence group quenched by a specific quencher. This peptide works as a substrate for 3CL Protease (MERS-CoV). After proteolytic cleavage, fluorescence product is formed and detected at λexcitation=485 nm and λemission=528 nm.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Kankanamalage A., et al., Eur. J. Med. Chem. 2018, 150: 334-346.

• Artikelnummer: BPS78278
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78278
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK