3CL Protease (Mpro), MBP-tag (SARS-CoV-2) Recombinant

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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Add 30 µl of 3CL protease and 10 µl of compound diluted in 1x assay buffer. Incubate 30 min at room temperature. Start the reaction by adding 10 µl of diluted substrate. Immediately place plate in plate reader. Read kinetics for 60 min (lexc=360 nm

Description: Severe acute respiratory Coronavirus 2 3C-like protease (SARS-CoV-2 3CL Protease), GenBank Accession No. YP_009725301, a.a. 1-306(full length), with an N-terminal MBP-tag, expressed in an E. coli expression system, MW=77.5 kDa.

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 8 mM maltose.

Genbank: YP_009725301

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal MBP-tag

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Muramatsu, T. et al., PNAS. 2016 Nov; 113(46):12997-13002.
2. Hsu, M.F. et al., JBC. 2005 Mar; 280:31257-31266.

• Artikelnummer: BPS100707-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100707-2
• Green Labware: Nein
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK