3CL Protease, Untagged (SARS-CoV-2) Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Great for studying enzyme kinetics and HTS applications.

Background: Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. CoVs may enter into the cells with the help of proteases such as Severe Acute Respiratory Syndrome Coronavirus 3C-like protease (SARS-CoV-1 3CL Protease). The 3CL protease, also known as Main Protease (Mpro), plays a vital role in processing the polyproteins that are translated from the viral RNA 3CL Protease inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the coronavirus infection.

Description: The Untagged 3CL Protease Assay Kit is designed to measure 3CL Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. The kit comes in a convenient 96-well format, with purified untagged 3CL Protease (BPS Bioscience #100823), fluorogenic substrate, and 3CL Protease assay buffer for 100 enzyme reactions. 3CL inhibitor GC376 is also included as a control.The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity, the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between glutamine and serine by the 3CL protease to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). EDANS has an excitation peak at 336 nm and an emission peak at 455 nm. The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience #79952).The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience #79952).

Storage Stability: At least one year from date of receipt when stored as directed.

Supplied As: The kit comes in a convenient 384-well format, with purified untagged 3CL Protease, fluorogenic substrate, and 3CL Protease assay buffer for 400 enzyme reactions. 3CL inhibitor GC376 is also included as a positive control.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Jared S. Morse, et al., 2020 Chem.Bio.Chem. 21:730-738.
2. Zhang, L., et al. 2020, Science 368 (6489): 409-412. 

• Artikelnummer: BPS78042-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78042-2
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK