4-Hydroxy-2-hexenal Antibody, Clone 5C11.1: RPE

Mouse Anti-4-Hydroxy-2-hexenal Monoclonal IgG1

Target: 4-Hydroxy-2-hexenal

Conjugate: RPE

Product Type: Monoclonal

Clone Number: 5C11.1

Immunogen: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)

Purification: Protein G Purified

Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated

Concentration: 1 mg/ml

Specificity: Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Scientific Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.

References: 1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSMC-536D-RPE
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SMC-536D-RPE
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Monoclonal
• Methode: Western Blotting, ELISA
• Isotyp: IgG1
• Wirt: Mouse