CAS No.: 61135-33-9.
Molecular Formula: C11H12N2O5.
Molecular Weight: 252,2.
Source: Synthetic.
Purity: >98% (HPLC); NMR (Conforms).
Solubility: May be dissolved in DMSO (10 mg/ml).
Appearance: Off-white powder.
PubChem CID: 472172.
Scientific Background: 5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine that is used to monitor DNA synthesis through click chemistry (1). Unlike 5-bromo-2’-deoxyuridine (BrdU), EdU does not require sample fixation or DNA denaturation. It can then be detected using a fluorescent azide probe and copper catalysis using 1,3-dipolar cycloaddition.
References: 1. Salic A., and Mitchison, T.J. (2008) Proc. Natl. Acad. Sci. USA. 105:2415.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.