A20, His tag, FLAG-tag (Sf9-derived) Recombinant

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Encompassing Amino Acids: 2-790(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Reaction was performedin 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mMEDTA, 500 nM Ub-AMC, and variousamount of A20 in a volume of 50 µL.Reaction was incubated at 37°C for 30 minand fluorescent signal was measured atexcitation of 360 nm, and emission at 460nm.

Description: Human A20, also known astumor necrosis factor- induced protein 3(TNFAIP3) or OTU domain-containingprotein 7C (OTUD7C), GenBank AccessionNo. NM_006290, amino-acids 2-790(end)with an N-terminal FLAG-tag and C-terminalHis-tag, MW=91 kDa, expressed in Sf9insect cells via a baculovirus expressionsystem.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol

Genbank: NM_006290

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag, C-terminal His-tag

Uniprot: Q9UHC1

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41

• Artikelnummer: BPS80408
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80408
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK