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Activated Protein C (PROC), His-Tag Recombinant
Activated Protein C (PROC), His-Tag Recombinant
Artikelnummer
BPS70019
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1-461 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl2, 0.01% Brij-35, 100 µM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460.
Description: Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 - 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 250 mM imidazole
Genbank: NM_000312
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P04070
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wagener, G., et al., Transplant. Proc. 2012,44(5):1336-1340.
2. Luo, L., et al., Mediators Inflamm. 2012,2012:485279.
Encompassing Amino Acids: 1-461 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl2, 0.01% Brij-35, 100 µM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460.
Description: Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 - 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 250 mM imidazole
Genbank: NM_000312
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P04070
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wagener, G., et al., Transplant. Proc. 2012,44(5):1336-1340.
2. Luo, L., et al., Mediators Inflamm. 2012,2012:485279.
| Artikelnummer | BPS70019 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 70019 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
