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Encompassing Amino Acids: 2-331 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Recombinant S. putrefaciens adenosine deaminase, or ADA, (GenBank Accession No. CP000681) a.a. 2- 331 (end) with N-terminal His-tag, MW= 37 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Genbank: CP000681
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: A4YCD7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Daddona, PE, et al., J Biol Chem. 1984 Oct 10; 259(19):12101-6.
2. Wang, H., et al., Heart Lung. 2012 Jan-Feb;41(1):70-5.