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101852 ARH3 (ADPRS), His-Tag Recombinant
101852 ARH3 (ADPRS), His-Tag Recombinant
Artikelnummer
BPS101852
Verpackungseinheit
25 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-363(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Various concentrations of ARH3 were incubated at room temperature with 4 µM TFMU-ADPr (substrate) and the fluorogenic product was measured after 1 hour (λexcitation: 385 nm / λemission: 502 nm).
Background: ADPRS, also known as ADP-ribosyl-acceptor hydrolase 3 or ARH3, is part of the DNA damage response machinery, and removes ADP-ribose from serine residues in a Mg2+-dependent manner. It acts sequentially to PARG (poly(ADP-ribose) glycohydrolase) and it has a protective role by decreasing the levels of PAR in the cell, which stops mitochondria from releasing PAR-driven AIF (apoptosis inducing factor). Mutations that result in loss of function of this protein lead to CONDSIAS (stress-induced childhood-onset neurodegeneration with variable ataxia and seizures), a disease with multiple clinical expressions. The development and use of ADPRS inhibitors allows us a better understanding of the DNA damage response pathway and opens new avenues for cancer treatment.
Description: Recombinant human ADPRS (ADP-ribosyl-acceptor hydrolase 3, or ARH3), full length encompassing amino acids 2-363(end). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Genbank: NM_017825.3
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag
Uniprot: Q9NX46
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
Encompassing Amino Acids: 2-363(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Various concentrations of ARH3 were incubated at room temperature with 4 µM TFMU-ADPr (substrate) and the fluorogenic product was measured after 1 hour (λexcitation: 385 nm / λemission: 502 nm).
Background: ADPRS, also known as ADP-ribosyl-acceptor hydrolase 3 or ARH3, is part of the DNA damage response machinery, and removes ADP-ribose from serine residues in a Mg2+-dependent manner. It acts sequentially to PARG (poly(ADP-ribose) glycohydrolase) and it has a protective role by decreasing the levels of PAR in the cell, which stops mitochondria from releasing PAR-driven AIF (apoptosis inducing factor). Mutations that result in loss of function of this protein lead to CONDSIAS (stress-induced childhood-onset neurodegeneration with variable ataxia and seizures), a disease with multiple clinical expressions. The development and use of ADPRS inhibitors allows us a better understanding of the DNA damage response pathway and opens new avenues for cancer treatment.
Description: Recombinant human ADPRS (ADP-ribosyl-acceptor hydrolase 3, or ARH3), full length encompassing amino acids 2-363(end). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Genbank: NM_017825.3
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag
Uniprot: Q9NX46
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101852 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101852 |
| Verpackungseinheit | 25 µg |
| Mengeneinheit | STK |
| Wirt | Escherichia Coli |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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