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AKT3, His-tag Recombinant
AKT3, His-tag Recombinant
Artikelnummer
BPS40012
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.
Description: Recombinant human AKT3 (GenBank Accession No. NM_005465), a.a. 2-end, with N-terminal His-tag, MW= 60 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_005465
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9Y243
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Coffer, PJ. et al. Biochem J. 1998 Oct 1; 335 (Pt 1):1-13.
2. Anderson, KE. et al. Curr Biol. 1998 Jun 4;8(12): 684-91.
Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.
Description: Recombinant human AKT3 (GenBank Accession No. NM_005465), a.a. 2-end, with N-terminal His-tag, MW= 60 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_005465
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9Y243
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Coffer, PJ. et al. Biochem J. 1998 Oct 1; 335 (Pt 1):1-13.
2. Anderson, KE. et al. Curr Biol. 1998 Jun 4;8(12): 684-91.
| Artikelnummer | BPS40012 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40012 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
