Conjugate: Alexa Fluor® 488
Physical State: Freeze-dried solid
Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Specificity: IgG2b, Fcγ fragment
Information: Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2b but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.