ALK , GST-tag Recombinant

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Encompassing Amino Acids: 1058-1620 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was done in a Kinase buffer using 0.2 mg/ml IGF1Rtide as a substrate with 20 µM ATP at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Description: Human anaplastic lymphoma receptor tyrosine kinase (ALK), also known as CD246 and NBLST3, (GenBank Accession No. NM_004304), a.a. 1058-1620 (end) with N-terminal GST-tag, MW = 89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol.

Genbank: NM_004304

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q9UM73

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ren, H., et al., Cancer Res. 2012,72(13):3312-3323.
2. Zhu, S., et al., Cancer Cell 2012,21(3):362-373.
3. Schonherr, C., et al., Crit Rev Oncog. 2012,17(2):123-143

• Artikelnummer: BPS40214
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40214
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect