ALK2 (ACVR1), FLAG-Tag, Avi-Tag, Biotin-Labeled Recombinant

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Encompassing Amino Acids: 147-509

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 µM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ALK2 incubated 45 minutes at 30 °C. Followed by addition of 25 µL of BPS ADP-Glo reagent incubated 45 minutes at room temperature. Followed by addition of 50 µl of BPS Kinase Detection Reagent incubated 45 minutes at room temperature. Luminescence was measured.

Description: Recombinant human ALK2 a.a. 147-509, (Genbank Accession No. NM_1001105) with an N-terminal FLAG-Tag and C-terminal Avi-Tag™, expressed in Sf9 cells using a baculovirus expression system. MW= 45 kDa.

Format: Aqueous buffer solution.

Formulation: Formulated in: 40mM Tris-HCl pH 8.0, 110mM NaCl, 2.2mM KCl, 8ng/µL Flag peptide, 3mM DTT, and 20% glycerol.

Genbank: NM_001105

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-Tag, C-terminal Avi-Tag

Uniprot: Q04771

Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Kaplan, F., S., et al., Hum. Mutat. 2009;30:379-390.2. Yu, P.B., et al., Nature Med. 2008;14:1363-1369.

• Artikelnummer: BPS100176
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100176
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK