ALKBH5 Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Background: ALKBH5 (AlkB Homolog 5, RNA Demethylase) is an enzyme that removes N6-Methyladenosine (m^6A) from target mRNAs. ALKBH5 has been connected to various cancers, viral replication, and metabolic diseases while also being shown to remove the alkylation damage and restore adenine in DNA.

Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt

Description: The ALKBH5 Chemiluminescent Assay Kit is designed to measure ALKBH5 activity for screening and profiling applications. The key to the ALKBH5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to ALKBH5 demethylase activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, ALKBH5 enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Storage Stability: At least 6 months from date of receipt, when stored as directed

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Zheng, G., Dahl, J.A., et al. 2013. ALKBH5 Is a Mammalian RNA Demethylase that Impacts RNA Metabolism and Mouse Fertility, Molecular Cell, 49(1):18-29.

• Artikelnummer: BPS79659
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79659
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK