AMPK (A1/B1/G3), His-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The γ subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism (2).

Description: Recombinant human AMPK (combination of A1/B1/G3 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 68 kDa (A1), 38 kDa (B1), 51 kDa (G3).

Format: Aqueous buffer solution

Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

Genbank: NM_006251 (A1), NM_006253 (B1), NM_017431 (G3)

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: A1: Q13131; B1: Q9Y478; G3: Q9UGI9

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216-219.
2. Cheung, P. C. F. et al: Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding. Biochem. J. 346: 659-669, 2000.

• Artikelnummer: BPS40702
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40702
• Green Labware: Nein
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK
• Wirt: Insect