AMPK (A2/ B1/G1), His-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2).

Description: Recombinant human AMPK (combination of A2/B1/G1 subunits), full length, with C-terminal His-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

Genbank: NM_006252 (A2), NM_006253 (B1), NM_002733 (G1)

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: A2: P54646; B1: Q9Y478; G1: P54619

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216-219.
2. Foretz, M. et al: Short-term overexpression of a constitutively active form of AMP-activated protein kinase in the liver leads to mild hypoglycemia and fatty liver. Diabetes, 2005; 54 (5);1331-1339.

• Artikelnummer: BPS40704
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40704
• Green Labware: Nein
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK
• Wirt: Insect