Anti-A2a (Fab2838)

Anti-A2a [Fab2838], Recombinant, Fab fragment kappa, Mouse
Artikelnummer
ABAAb04600-1.29-BT
Verpackungseinheit
500 μg
Hersteller
Absolute Antibody

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CloneID: Fab2838

Heavy Chain modification: His-Tagged

Antigen Long Description: The original antibody was raised by immunizing a mouse with purified A2AAR-antagonist ZM241385 complex 3.

Buffer Composition: PBS only.

Uniprot Accession No.: P29274

Specificity Statement: This antibody is specific for the intracellular surface of A(2A)AR.

Application Notes (Clone): The original antibody was used for an ELISA, where it showed it was able to bind its antigen. The antibody was used for a ligand binding assay. Here it showed that the antibody was able to stop the binding of agonist NECA but not that of antagonist ZM241385. This suggests that this antibody induces a inactive confirmation of A2AAR. The structure of the original antibody was determined using x-ray crystallography (Hino et al., 2012; PMID:22286059). The heavy chain of this antibody was cloned into a plasmid and HEK293T were transfected. In vitro is showed it was able to block the A2AR signalling pathway (Zhang et al., 2019; PMID:32308059). The protein-protein interaction of this antibody with its antigen was assessed using mCBB CN-PAGE (Suzuki et al., 2018; PMID:29432751).
Mehr Informationen
Artikelnummer ABAAb04600-1.29-BT
Hersteller Absolute Antibody
Hersteller Artikelnummer Ab04600-1.29-BT
Verpackungseinheit 500 μg
Mengeneinheit STK
Reaktivität Human
Klonalität Recombinant
Methode ELISA, Blocking
Isotyp Fab fragment kappa
Wirt Mouse
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