Anti-A2a (Fab2838)

Anti-A2a [Fab2838], Recombinant, IgG kappa, Rabbit

CloneID: Fab2838

Antigen Long Description: The original antibody was raised by immunizing a mouse with purified A2AAR-antagonist ZM241385 complex 3.

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P29274

Specificity Statement: This antibody is specific for the intracellular surface of A(2A)AR.

Application Notes (Clone): The original antibody was used for an ELISA, where it showed it was able to bind its antigen. The antibody was used for a ligand binding assay. Here it showed that the antibody was able to stop the binding of agonist NECA but not that of antagonist ZM241385. This suggests that this antibody induces a inactive confirmation of A2AAR. The structure of the original antibody was determined using x-ray crystallography (Hino et al., 2012; PMID:22286059). The heavy chain of this antibody was cloned into a plasmid and HEK293T were transfected. In vitro is showed it was able to block the A2AR signalling pathway (Zhang et al., 2019; PMID:32308059). The protein-protein interaction of this antibody with its antigen was assessed using mCBB CN-PAGE (Suzuki et al., 2018; PMID:29432751).

• Artikelnummer: ABAAb04600-23.0
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04600-23.0
• Verpackungseinheit: 100 μg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Recombinant
• Methode: ELISA, Blocking
• Isotyp: IgG kappa
• Wirt: Rabbit