CloneID: Lca12
Antigen Long Description: This antibody was generated by panning against purified virus using a antibody library from five different 5-week old white leghorn hens.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for the african horse sickness virus. It is the pathogen that causes african horse sickness in horses, mules and donkeys.
Application Notes (Clone): To show that this antibody can detect virus particles, a sandwich ELISA was performed on African horse sickness virus, it showed a positive result (van Wyngaardt et al, 2004; pmid: 15059288). A DAS-ELISA was performed on the scFv version of this antibody using AHSV-3 as antigen, it showed a limit of detection of 2 ng and reacted very strongly to the AHSV-3 serotype and very little with other serotypes (van Wyngaardt et al, 2013; pmid:23388433).
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