CloneID: scFv59
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was isolated from a human scFv phage library by screening against Abeta1-42.
Origin Pub PMID: 16630540
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P05067
Specificity Statement: This antibody is specific for oligomeric as well as fibrillar/aggregated Aβ.
Application Notes (Clone): The scFv version of this antibody demonstrated the highest ELISA titer for Aβimmunoreactivity against Aβ1–42, and its specificity for oligomeric and fibrillar/aggregated Aβ was demonstrated in a WB. The scFv version of this antibody reacted with amyloid beta deposits in brain sections from a Tg2576 mouse and in skeletal muscle sections from a Tg13592 mouse, as observed via IHC. The scFv version of this antibody was used to inhibit the formation of Aβ fibril formation in vitro as demonstrated in a Thioflavin T assay. This inhibition contributed to the amelioration of Aβ cytotoxicity as was shown in vitro in an MTT assay; cell viability increased from 45.2 ± 0.78% to 58.8 ± 5.45% (P = 0.069) in the presence of scFv59. Furthermore, Tg2576 mice subjected to scFv59 injection in the hippocampus and cortex had fewer diffuse and fibrillar Aβ deposits in those areas than Tg2576 mice injected with PBS (Fukuchi et al., 2006; PMID: 16630540). Vascular amyloid was also stained by scFv59 (Fukuchi et al., 2006; PMID: 16766200).
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