Products from BPS Bioscience require a minimum order value above 400€
Applications: WB, DB, ELISA, ICC, FC
Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Clone Number: clone BA3R
Cross Reactivity: Predicted to work with: Sheep, Horse, Guinea pig, Hamster, Cow, Pig, Chimpanzee, Cynomolgus Monkey, Japanese ricefish, Orangutan
Description: Affinity purified Monoclonal Anti-β-Tag, clone BA3R, mouse IgG2b. Recognizes native and denatured forms of β-Actin (~42kDa). Applications: Western Blot(1:1000-10000) ,Dot Blot, ELISA, Immunocytochemistry(1:500-2000), Flow cytometry (0.1-1 µg for 10(6) cells). Optimal dilutions/concentrations should be determined by the end user.
Format: Aqueous buffer solution
Formulation: 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen: Synthetic peptide corresponding to a.a. 2 - 15 (DDDIAALVVDNGSG) of Human beta Actin cytoplasmic domain
Purification: Immunogen affinity purified
Storage Stability: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)