Anti-CD123 Antibody, Biotin-Labeled

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Application: Useful for binding of CD123 in ELISA and in cellular assays.

Assay Conditions: The antibody was validated by measuring anti-CD123 binding to CD123 antigen by ELISA. CD123, Avi-His-Tag Recombinant (BPS Bioscience #101035) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at Room Temperature (RT). After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CD123 antibody (BPS Bioscience #101733), serially diluted in Blocking Buffer 2, was added for 30 minutes at RT. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Background: CD123, also known as interleukin 3 receptor alpha (IL3Ra), is a transmembrane protein found in pluripotent progenitor cells and involved in hematopoietic lineage differentiation. It can also be found in basophils, plasmacytoid dendritic cells and dendritic cells. CD123 is overexpressed on leukemic hematopoietic stem cells (HSCs), but not normal HSCs. It has been found in acute myeloid and B-lymphoid leukemias, blastic plasmacytoid dendritic neoplasms (BPDCN) and hairy cell leukemia. This expression profile makes CD123 an attractive target in cancer therapy. CAR-Ts and other therapies targeting CD123 are currently being evaluated in clinical trials.

Description: This anti-CD123 antibody is a purified, recombinant human monoclonal antibody that recognizes human CD123. This construct contains an Avi-tag™ in the middle of the Heavy Chain constant region. This recombinant antibody has been tested for specific binding to purified recombinant human CD123 by ELISA.

Format: Aqueous buffer solution.

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Host Cell Line: HEK293

Purity: ≥90%

Storage Stability: At least 12 months at -80°C.

Uniprot: O75794

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101733-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101733-2
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Methode: ELISA
• Isotyp: IgG1
• Wirt: Human
• Konjugat: Conjugated, Biotin