Anti-CD16a Antibody, FITC-Labeled

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Application: Useful for studying expression of CD16a on the cell surface by flow cytometry

Assay Conditions: One million CD16a CHO cells (BPS Bioscience #78332) were blocked and stained with 1 µg of anti-CD16a antibody, FITC-labeled (BPS Bioscience #101979) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD16a CHO cells (green) were compared to parental CHO-K1 cells (blue). The Y-axis represents the cell count while the X-axis shows FITC intensity.

Background: CD16a, also known as Fc Gamma Receptor 3A (FcGRIIIA; FcγRIIIA), is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, antibody-dependent cytotoxicity, and clearance of immune complexes. The human FcGR3A displays a dimorphism at residue 158. The allele V158 encodes a higher Fc affinity receptor variant while the allele F158 encodes a lower Fc affinity receptor variant. FcGR3A (CD16a) plays a role in the activation of natural killer (NK) cells, and it is extensively used as a cell marker. Its expression has also been observed at the surface of T cells in patients with chronic viral infections (such as COVID-19). Binding to CD16a can potentiate the efficacy of therapeutic antibodies used to treat solid tumors. In addition, CD16a is a biomarker in hematopoietic cancers and  an interesting therapeutic target.

Cross Reactivity: This antibody recognizes human CD16a. It has not been tested on other species

Description: This human anti-CD16a antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for binding to human CD16a on the surface of the CD16a CHO Recombinant Cell Line (BPS Bioscience #78332).

Format: Aqueous buffer solution.

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

Host Cell Line: HEK293

Purification: Protein A affinity chromatography from HEK293 supernatant

Purity: ≥90%

Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Uniprot: P08637

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101979-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101979-2
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Isotyp: Human IgG1
• Wirt: Human
• Konjugat: Conjugated, FITC