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Anti-CD19-Anti-CD3 IgG format Bispecific Antibody
Anti-CD19-Anti-CD3 IgG format Bispecific Antibody
Artikelnummer
BPS101076-2
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
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Assay Conditions: Experimental design and assay protocol for measuring anti CD19-anti CD3 functional activity using NFAT-luc reporter Jurkat cell line:Jurkat effector cells with endogenous TCR/CD3 and transfected reporter NFAT-luc (BPS Bioscience #60621) are incubated with increasing concentrations of anti-CD19 x anti-CD3 bispecific antibody in the presence of CD19-CHO cells (BPS Bioscience #79561-H) or CHO cells (ATCC #CCL-61™).
1. Seed CHO and CD19-CHO cells at 30,000 cells/well and allow a few hours for the cells to attach in a 96-well clear bottom white plate.
2. Seed Jurkat cells at 30,000 cells/well.
3. Add the bispecific antibody at a recommended dilution range of 100 fM-100 nM. The bispecific antibody simultaneously binds to TCR/CD3 on the NFAT-luc Jurkat reporter cells and tumor antigen CD19 on CD19-CHO cells.
4. After 16 hours the luciferase activity is measured using ONE-Step™ luciferase assay (BPS Bioscience #60690) per recommended protocol. The bispecific antibody interaction stimulates NFAT-luciferase activity.
Description: Anti-CD19-Anti-CD3 IgG format bispecific antibody is a purified recombinant human bispecific antibody with T cell Engager. This bispecific antibody has been tested for specific activity in the functional reporter assay using NFAT-luc reporter Jurkat cell line (BPS Bioscience #60621) in the presence of CD19-CHO cells (BPS Bioscience #79561-H) that overexpress CD19.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Purification: Protein A affinity purification of the IgG-tag protein from HEK293 cells.
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Assay Conditions: Experimental design and assay protocol for measuring anti CD19-anti CD3 functional activity using NFAT-luc reporter Jurkat cell line:Jurkat effector cells with endogenous TCR/CD3 and transfected reporter NFAT-luc (BPS Bioscience #60621) are incubated with increasing concentrations of anti-CD19 x anti-CD3 bispecific antibody in the presence of CD19-CHO cells (BPS Bioscience #79561-H) or CHO cells (ATCC #CCL-61™).
1. Seed CHO and CD19-CHO cells at 30,000 cells/well and allow a few hours for the cells to attach in a 96-well clear bottom white plate.
2. Seed Jurkat cells at 30,000 cells/well.
3. Add the bispecific antibody at a recommended dilution range of 100 fM-100 nM. The bispecific antibody simultaneously binds to TCR/CD3 on the NFAT-luc Jurkat reporter cells and tumor antigen CD19 on CD19-CHO cells.
4. After 16 hours the luciferase activity is measured using ONE-Step™ luciferase assay (BPS Bioscience #60690) per recommended protocol. The bispecific antibody interaction stimulates NFAT-luciferase activity.
Description: Anti-CD19-Anti-CD3 IgG format bispecific antibody is a purified recombinant human bispecific antibody with T cell Engager. This bispecific antibody has been tested for specific activity in the functional reporter assay using NFAT-luc reporter Jurkat cell line (BPS Bioscience #60621) in the presence of CD19-CHO cells (BPS Bioscience #79561-H) that overexpress CD19.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Purification: Protein A affinity purification of the IgG-tag protein from HEK293 cells.
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101076-2 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101076-2 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Klonalität | Monoclonal |
| Isotyp | Human IgG1 |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
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