Anti-CD20 Antibody, PE-Labeled

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Applications: Useful for studying expression of CD20 on the cell surface in flow cytometry assay. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Assay Conditions: One million CD20 CHO recombinant cells (BPS Bioscience #79624>) were blocked and stained with 1 µg of anti-CD20 antibody, PE-labeled (BPS Bioscience #101672) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD20 CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.

Concentration: The concentration will vary with each lot; the exact value will be provided with each shipment.

Cross Reactivity: This antibody recognizes human CD20. It has not been tested with other species.

Description: This anti-CD20 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested in flow cytometry assay for specific binding to human CD20 on the surface of CD20 CHO Recombinant Cell Line (BPS Bioscience #79624).

Format: Aqueous buffer solution

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain protein stabilizer.

Host Cell Line: HEK293

Purification: Protein A affinity chromatography from HEK293 supernatants.

Purity: ≥90%

Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C. 

Uniprot: P11836

Warnings: Do not freeze. Protect from light.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101672-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101672-2
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Isotyp: IgG1
• Wirt: Human