Anti-CD20 IgG Antibody, Biotin-labeled

Products from BPS Bioscience require a minimum order value above 400€

Applications: This product is for research use only. It is not suitable for human diagnostic or therapeutic use.

Assay Conditions: Functional validation: The antibody was validated by ELISA using a purified CD20 antigen. The CD20 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CD20 antibody (BPS Bioscience #101207), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.   

Description: Biotinylated anti-CD20 IgG antibody is a purified recombinant antibody which recognizes human CD20 antigen. This antibody has been tested for specific binding to purified human CD20 protein.

Format: Aqueous buffer solution

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Purification: Protein A affinity

Purity: ≥90%

Storage Stability: At least 12 months at -80°C. 

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101207-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101207-2
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Isotyp: Human IgG1
• Wirt: Human