CloneID: DC1.7
Antigen Long Description: The original antibody was isolated from the peripheral blood lymphocytes of a human patient with a history of symptomatic CHIKV infection using single B cell sorting with biotinylated p62-E1 hybrid protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q8JUX5
Specificity Statement: This antibody binds and epitope near the E1 DIII domain of the Chikungunya virus (CHIKV), which is the causative agent of zoonotic disease in humans called Chikungunya. Additionally, this antibody also cross reacts with multiple arthritogenic alphaviruses like Mayaro virus (MAYV), Ross River (RRV) and Sindbis virus (SINV) (PMID: 34416146). CHIKV belongs to the alphavirus genus of the Togaviridae family and is transmitted to human by infected mosquito bites. A CHIKV infection causes fever and severe joint pain. Other symptoms include muscle pain, joint swelling, headache, nausea, fatigue and rash. The disease mostly occurs in Africa, Asia and the Indian subcontinent.
Application Notes (Clone): The binding characterization of the IgG antibody for CHIKV E1 protein was done using ELISA and Biolayer interferometry (BLI). This antibody was capable of binding biotinylated p62-E1 hybrid protein in a flow cytometric assay. This antibody was also capable of immunoprecipitating the E1 envelope protein of CHIKV. This antibody was can neutralize CHIKV 181/25 and CHIKV LR2006_OPY1 with IC50 value in the low nanomolar range and 13nM respectively. This antibody could not provide sufficient in vivo protection from CHIKV infection (PMID: 31697791).
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