Antigen Long Description: The original antibody was generated by sequencing the total mRNA obtained from murine 10B5 E7E2 hybridoma. Recombinant 10B5 scFv–M13 phage were rescued from the clones and panned against WEE antigen immobilized on PVC 96-well plates. Later on mouse/human IgG1 chimeric version of the antibody was generated by using variable regions derived from the mouse 10B5 hybridoma and human Fc regions.