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Anti-ER alpha Monoclonal Antibody
Anti-ER alpha Monoclonal Antibody
Artikelnummer
BPS25221
Verpackungseinheit
100 µl
Hersteller
BPS Bioscience
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Applications: ChIP/ChIP - seq (2.5 µl)
ELISA (1:500 - 1:5000)
WB (1:500 - 1:5000)
Gel Supershift (1:10 - 1:20)
Immunochemistry (1:500 - 1:5000)
IP (1:200 - 1:5000 )
Assay Conditions: ChIP-seq results obtained with the monoclonal antibody directed against ERalpha
ChIP was performed with the monoclonal antibody against ERalpha (Cat. # 25221) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The immunoprecipitated DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).
Background: The estrogen receptor alpha (ERalpha, UniProt/Swiss-Prot entry P03372) belongs to the family of nuclear hormone receptors, which are ligand-activated transcription factors. They are important for the regulation of gene expression, cellular proliferation and differentiation, sexual development and reproductive function. Estrogen receptors are also involved in pathological processes such as breast cancer, and osteoporosis. ERalpha can regulate transcription by direct binding to estrogen response elements (EREs) in the DNA or by interaction with other transcription factors. It may also form a heterodimer with ERbeta.
Concentration: 100 µl
Cross Reactivity: Does not react with chicken; other species not tested
Description: Monoclonal antibody raised in mouse against human ERalpha (estrogen receptor alpha), using a synthetic peptide
Format: Aqueous solution
Formulation: Ascites fluid from mouse containing 0.05% azide
Immunogen: synthetic peptide
Purification: Ascites fluid
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Applications: ChIP/ChIP - seq (2.5 µl)
ELISA (1:500 - 1:5000)
WB (1:500 - 1:5000)
Gel Supershift (1:10 - 1:20)
Immunochemistry (1:500 - 1:5000)
IP (1:200 - 1:5000 )
Assay Conditions: ChIP-seq results obtained with the monoclonal antibody directed against ERalpha
ChIP was performed with the monoclonal antibody against ERalpha (Cat. # 25221) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The immunoprecipitated DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).
Background: The estrogen receptor alpha (ERalpha, UniProt/Swiss-Prot entry P03372) belongs to the family of nuclear hormone receptors, which are ligand-activated transcription factors. They are important for the regulation of gene expression, cellular proliferation and differentiation, sexual development and reproductive function. Estrogen receptors are also involved in pathological processes such as breast cancer, and osteoporosis. ERalpha can regulate transcription by direct binding to estrogen response elements (EREs) in the DNA or by interaction with other transcription factors. It may also form a heterodimer with ERbeta.
Concentration: 100 µl
Cross Reactivity: Does not react with chicken; other species not tested
Description: Monoclonal antibody raised in mouse against human ERalpha (estrogen receptor alpha), using a synthetic peptide
Format: Aqueous solution
Formulation: Ascites fluid from mouse containing 0.05% azide
Immunogen: synthetic peptide
Purification: Ascites fluid
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS25221 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 25221 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µl |
| Mengeneinheit | STK |
| Reaktivität | Human |
| Klonalität | Monoclonal |
| Methode | Immunoprecipitation, Western Blotting, ELISA, Chromatin Immunoprecipitation (ChIP) |
| Produktinformation (PDF) |
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| MSDS (PDF) |
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Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
